Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference
نویسندگان
چکیده
منابع مشابه
Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference.
RNA interference (RNAi) is an ancient antiviral response that processes dsRNA and associates it into a nuclease complex that identifies RNA with sequence homology and specifically cleaves it. We demonstrate that RNAi mediated by 21-bp dsRNA specifically inhibits HIV-1 infection of permanent cell lines and primary CD4(+) T cells. Inhibition of HIV replication was measured by p24 Gag protein cont...
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Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...
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Double-stranded RNAs approximately 21 nucleotides long [small interfering RNA (siRNA)] are recognized as powerful reagents to reduce the expression of specific genes. To use them as reagents to protect cells against viral infection, effective methods for introducing siRNAs into primary cells are required. Here, we describe success in constructing a lentivirus-based vector to introduce siRNAs ag...
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ژورنال
عنوان ژورنال: The Journal of Immunology
سال: 2002
ISSN: 0022-1767,1550-6606
DOI: 10.4049/jimmunol.169.9.5196